Survey Form

<body topmargin=0 leftmargin=0 marginheight=0 marginwidth=0> <table cellpadding=0 cellspacing=0 border=0><tr> <td valign="top" colspan=2 height=82 BGCOLOR="#111111" TEXT="#F7F7FF"> <div> <FONT SIZE="5" COLOR="#33CCFF" FACE="Comic Sans MS" ><B>Seven  Creeks  Roadside  Spring Water</B></FONT><B>     |     </B><A HREF="index.htm" TARGET="_top" TITLE="Seven Creeks Roadside Spring Water"><B>home</B></A></div> <div> <A HREF="id278.htm" TARGET="_top" TITLE="Watershed Critters"><B><IMG SRC="0720e0e0.png" border=0 width="14" height="14" ALT="up" ALIGN="BOTTOM" HSPACE="0" VSPACE="0"></B></A><B>  </B><IMG BORDER="0" SRC="1x1.gif" HEIGHT="14" ALIGN="BOTTOM" HSPACE="0" VSPACE="0" WIDTH="14"><B>    </B><A HREF="id308.htm" TARGET="_top" TITLE="Mallards"><B><IMG SRC="0710e0e0.png" border=0 width="14" height="14" ALT="previous" ALIGN="BOTTOM" HSPACE="0" VSPACE="0"></B></A><B>  </B><A HREF="id455.htm" TARGET="_top" TITLE="Trilliums"><B><IMG SRC="0730e0e0.png" border=0 width="14" height="14" ALT="next" ALIGN="BOTTOM" HSPACE="0" VSPACE="0"></B></A></div> <div> </div> </td> </tr><tr> <td valign="top" width=450 BGCOLOR="#111111" TEXT="#F7F7FF"> <div> <I>Lesson 1- Introductory Lesson-Determination of Overall Water Quality Using a Quantitative Macroinvertebrate Survey</I></div> <bR> <div> <B>Objectives:</B> </div> <div> Students will be able to: </div> <div> -identify various types of macroinvertebrates found in water ecosystems. </div> <div> -perform a quantitative macroinvertebrate survey to determine the overall water quality of a water ecosystem. </div> <div> <B>Materials:</B> </div> <div> Water sample from a local stream or pond (sample must be fresh and contain debris from the bottom and edges of the water) </div> <div> Macroinvertebrate Identification charts </div> <div> Assorted trays and petri dishes for separation of sample </div> <div> Dissecting microscope and hand lenses </div> <div> Small paintbrushes </div> <div> Pipets </div> <div> <B>Procedure:</B> </div> <div> 1. Working with a partner, take an approximate 500 ml sample of the water including the debris. </div> <div> 2. Pour a portion of the sample into a petri dish and examine it under low power on the microscope. </div> <div> 3. Using the identification charts, count and identify 100 macroinvertebrates. Try not to hunt for only the large ones or your sample will be biased. If you can not find 100 macroinvertebrates in your sample, take an additional 500 ml sample. Continue this procedure until you and your partner have counted and indentified 100 macroinvertebrates. </div> <div> 4. Identify and count the numbers of each type of organism listed below. </div> <div> 5. Multiply the number of each type by its biotic value. </div> <div> 6. Add up all the numbers and divide by 10. This gives the Biotic Index Value. Check the Biotic Value chart at the bottom of the page to determine the quality of your water sample. </div> <div> 7. Compare your group value with the class values. Do they agree? Why or why not? </div> <bR> <bR> <div> <B>Class I Pollution intolerant: These organisms are highly sensitive to pollution.</B> </div> <div> <B>Class II Somewhat pollution tolerant: These organisms will be found in clean and slightly polluted waterways.</B> </div> <div> <B>Class III Pollution tolerant: These organisms will be found in polluted, as well as clean aquatic ecosystems.</B> </div> <div> <TABLE BORDER="2" CELLPADDING="1" CELLSPACING="1" WIDTH="656" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="BOX" RULES="ALL" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=CENTER HEIGHT= 46 WIDTH="93"><div> Class I </div> </tD> <tD VALIGN=CENTER><NOBR><div> Biotic Value</div> </NOBR></tD> <tD VALIGN=CENTER><NOBR><div> Number</div> <div> Found</div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="93"><div> Class II </div> </tD> <tD VALIGN=CENTER><NOBR><div> Biotic </div> <div> Value</div> </NOBR></tD> <tD VALIGN=CENTER><NOBR><div> Number </div> <div> Found</div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="93"><div> Class III </div> </tD> <tD VALIGN=CENTER><NOBR><div> Biotic </div> <div> Value</div> </NOBR></tD> <tD VALIGN=CENTER><NOBR><div> Number </div> <div> Found</div> </NOBR></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Stonefly nymph </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Beetle larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="50"><div> 8</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Midge fly larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="50"><div> 5</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Mayfly nymph </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Sowbug </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 8</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Snails </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 4</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Dobsonfly larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Scud </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Leech </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 2</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 33 ><NOBR><div> Caddisfly larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Clams, Mussels </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Aquatic worms </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="50"><div> 0</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Riffle beetle </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Crayfish </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Water penny </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="84"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Cranefly larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 33 WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="84"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="84" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Dragonfly nymph </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 33 WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="84"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="84" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Damselfly nymph </div> </tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="33" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="84"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="84" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Black fly larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="50"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 WIDTH="93"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="84"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="84" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="43"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="43" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="50"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="50" HSPACE="0" VSPACE="0"></tD> </tR> </TABLE></div> <div> <B>For example if you find:</B> </div> <div> <B>25 mayflies 25 x 10 = 250</B> </div> <div> <B>15 caddisflies 15 x 10 = 150</B> </div> <div> <B>20 stoneflies 20 x 10 = 200</B> </div> <div> <B>20 scuds 20 x 6 = 120</B> </div> <div> <B>20 midge larva 20 x 5 = 100</B> </div> <div> <B>Total = 820 / 10 = 82</B> </div> <div> <B>Based on the Biotic Index value the water quality is excellent.</B> </div> <bR> <div> <B>Biotic Index</B></div> <div> Excellent >70</div> <div> Good 60-79</div> <div> Fair 40-59</div> <div> Poor <40</div> <bR> <div> ~ajclevel/macroinvert.html"~ajclevel/macroinvert.html"<B>~ajclevel/macroinvert.html"</B><FONT SIZE="3" COLOR="#0000FF" FACE="Arial" ><B><U>Return to Water Quality and Macroinvertebrates</U></B></FONT> </div> <bR> <bR> <bR> <bR> <div> <TABLE BORDER="0" CELLPADDING="1" CELLSPACING="1" WIDTH="626" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="VOID" RULES="NONE" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=CENTER HEIGHT= 16 WIDTH="622"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="16" ALIGN="bottom" WIDTH="622" HSPACE="0" VSPACE="0"></tD> </tR> </TABLE></div> <bR> <div> .</div> </td> <td valign="top" height=398 width="100%" BGCOLOR="#111111" TEXT="#F7F7FF"> <div> <I>Survey Form</I></div> <div> This  <A NAME="survey_form"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="1" ALIGN="bottom" WIDTH="1" HSPACE="0" VSPACE="0" ></A>survey form is used to determine water quality by sampling for creek critters and is from <A HREF="http://www4.ncsu.edu/~ajclevel/macroinvert.html" TARGET="_top" TITLE="http://www4.ncsu.edu/~ajclevel/macroinve"><B>http://www4.ncsu.edu/~ajclevel/macroinvert.html</B></A>. This information is similar to the one used by the students in the stream quality assesment for used in their field trip. </div> <bR> <div> <I>Lesson 1- Introductory Lesson-Determination of Overall Water Quality Using a Quantitative Macroinvertebrate Survey (creek critters)</I></div> <bR> <div> <B>Objectives:</B> </div> <div> Students will be able to: </div> <div> -identify various types of macroinvertebrates found in water ecosystems. </div> <div> -perform a quantitative macroinvertebrate survey to determine the overall water quality of a water ecosystem. </div> <div> <B>Materials:</B> </div> <div> Water sample from a local stream or pond (sample must be fresh and contain debris from the bottom and edges of the water) </div> <div> Macroinvertebrate Identification charts </div> <div> Assorted trays and petri dishes for separation of sample </div> <div> Dissecting microscope and hand lenses </div> <div> Small paintbrushes </div> <div> Pipets </div> <div> <B>Procedure:</B> </div> <div> 1. Working with a partner, take an approximate 500 ml sample of the water including the debris. </div> <div> 2. Pour a portion of the sample into a petri dish and examine it under low power on the microscope. </div> <div> 3. Using the identification charts, count and identify 100 macroinvertebrates. Try not to hunt for only the large ones or your sample will be biased. If you can not find 100 macroinvertebrates in your sample, take an additional 500 ml sample. Continue this procedure until you and your partner have counted and indentified 100 macroinvertebrates. </div> <div> 4. Identify and count the numbers of each type of organism listed below. </div> <div> 5. Multiply the number of each type by its biotic value. </div> <div> 6. Add up all the numbers and divide by 10. This gives the Biotic Index Value. Check the Biotic Value chart at the bottom of the page to determine the quality of your water sample. </div> <div> 7. Compare your group value with the class values. Do they agree? Why or why not? </div> <bR> <bR> <div> <B>Class I Pollution intolerant: </B>These organisms are highly sensitive to pollution. </div> <div> <B>Class II Somewhat pollution tolerant: </B>These organisms will be found in clean and slightly polluted waterways. </div> <div> <B>Class III Pollution tolerant: </B>These organisms will be found in polluted, as well as clean aquatic ecosystems. </div> <div> <TABLE BORDER="2" CELLPADDING="1" CELLSPACING="1" WIDTH="682" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="BOX" RULES="ALL" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><div> Class I </div> </tD> <tD VALIGN=CENTER WIDTH="65"><div> Biotic </div> <div> Value</div> </tD> <tD VALIGN=CENTER><NOBR><div> Number</div> <div> Found</div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="93"><div> Class II </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> Biotic </div> <div> Value</div> </tD> <tD VALIGN=CENTER><NOBR><div> Number </div> <div> Found</div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="93"><div> Class III </div> </tD> <tD VALIGN=CENTER><NOBR><div> Biotic </div> <div> Value</div> </NOBR></tD> <tD VALIGN=CENTER><NOBR><div> Number </div> <div> Found</div> </NOBR></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><div> Stonefly nymph </div> </tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER><NOBR><div> Beetle larva </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="61"><div> 8</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Midge fly larva </div> </tD> <tD VALIGN=CENTER WIDTH="45"><div> 5</div> </tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Mayfly nymph </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Sowbug </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 8</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Snails </div> </tD> <tD VALIGN=CENTER WIDTH="45"><div> 4</div> </tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><div> Dobsonfly larva </div> </tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Scud </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Leech </div> </tD> <tD VALIGN=CENTER WIDTH="45"><div> 2</div> </tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><div> Caddisfly larva </div> </tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Clams, Mussels </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Aquatic worms </div> </tD> <tD VALIGN=CENTER WIDTH="45"><div> 0</div> </tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 ><NOBR><div> Riffle beetle </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Crayfish </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 ><NOBR><div> Water penny </div> </NOBR></tD> <tD VALIGN=CENTER WIDTH="65"><div> 10</div> </tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Cranefly larva </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="99" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="65"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="65" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Dragonfly nymph </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="99" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="65"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="65" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Damselfly nymph </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 38 WIDTH="99"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="99" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="65"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="65" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Black fly larva </div> </tD> <tD VALIGN=CENTER WIDTH="61"><div> 6</div> </tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="93" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="38" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> <tR> <tD VALIGN=CENTER HEIGHT= 19 WIDTH="99"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="65"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="65" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="58"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="58" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="61"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="61" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="62"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="62" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="93"><div> Total </div> </tD> <tD VALIGN=CENTER WIDTH="45"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="45" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=CENTER WIDTH="56"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="56" HSPACE="0" VSPACE="0"></tD> </tR> </TABLE></div> <div> <B>Biotic Index   </B>Excellent >70    Good 60-79    Fair 40-59    Poor <40</div> <bR> <bR> <div> For example: </div> <div> 25 mayflies 25 x 10 = 250 </div> <div> 15 caddisflies 15 x 10 = 150 </div> <div> 20 stoneflies 20 x 10 = 200 </div> <div> 20 scuds 20 x 6 = 120 </div> <div> 20 midge larva 20 x 5 = 100 </div> <div> Total = 820 / 10 = 82 </div> <div> Based on the Biotic Index value the water quality is excellent. </div> <bR> <bR> <bR> <bR> <bR> <bR> <div> <TABLE BORDER="0" CELLPADDING="1" CELLSPACING="1" WIDTH="626" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="VOID" RULES="NONE" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=CENTER HEIGHT= 19 WIDTH="622"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="622" HSPACE="0" VSPACE="0"></tD> </tR> </TABLE></div> <bR> <bR> </td> </tr></table></body>